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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
11/05/2023 |
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Actualizado : |
11/05/2023 |
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Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
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Autor : |
CIUFFO, C.; ARÉVALO, A.P.; GARCIA, F.; PERDOMO, T.; CRISPO, M.; GIANNITTI, F.; BUSCHIAZZO, A.; ZARANTONELLI, L. |
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Afiliación : |
CAMILA CIUFFO, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; ANA PAULA ARÉVALO, Unidad de Biotecnología en Animales de Laboratorio.; FEDERICO GARCIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; TERESITA YISELL PERDOMO TORRES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALEJANDRO BUSCHIAZZO, Laboratorio de Microbiología Molecular y Estructural, Institut Pasteur de Montevideo, Montevideo, Uruguay; LETICIA ZARANTONELLI, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; Laboratorio de Microbiología Molecular y Estructural, Institut Pasteur de Montevideo, Montevideo, Uruguay. |
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Título : |
Estudio y caracterización de una variante autóctona de Leptospira para su potencial uso como antígeno en vacunas de uso veterinario. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
In: Jornadas Uruguayas de Buiatría, 49., 2022. Paysandú, Uruguay: Centro Médico Veterinario de Paysandú; Filial de la Sociedad de Medicina Veterinaria del Uruguaya; Sociedad Uruguaya de Buiatría, 9 al 11 Junio 2022. p.192-194. |
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Páginas : |
p.192-194. |
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Idioma : |
Español |
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Contenido : |
RESUMEN: En Uruguay, Leptospira interrogans serogrupo Pomona serovar Kennewicki es una de las variantes más frecuentemente aislada en bovinos. Las vacunas actualmente disponibles contra leptospirosis bovina no incluyen esta variante autóctona. En este trabajo evaluamos la capacidad infectiva e inmunogénica de la variante autóctona de L. interrogans Pomona Kennewicki aislada de bovinos, en un modelo murino de infección sub-letal y crónica utilizando una vía de infección natural. Los resultados obtenidos demuestran la capacidad infectiva, de diseminación sanguínea, colonización del parénquima renal e inmunogenicidad de este aislamiento autóctono validando así su potencial uso como antígeno de interés a ser incluido en la formulación de vacunas anti-leptospira de uso veterinario en Uruguay y la región. --------------- SUMMARY: Leptospira interrogans serogroup Pomona serovar Kennewicki is one of the most frequently isolated variants in cattle in Uruguay. Currently available vaccine against bovine leptospirosis do not include this autochthonous strain. In this work we evaluated the infective and immunogenic capacity of the autochthonous variant of L.
interrogans Pomona Kennewicki isolated from bovines, in a chronic sublethal murine model using a natural route of infection. The results demonstrate the infective capacity, blood dissemination, colonization of the renal parenchyma and immunogenicity of this autochthonous isolate, thus validating its potential use as an antigen of interest to be included in the formulation of anti-leptospira vaccines for veterinary use in Uruguay and the region. MenosRESUMEN: En Uruguay, Leptospira interrogans serogrupo Pomona serovar Kennewicki es una de las variantes más frecuentemente aislada en bovinos. Las vacunas actualmente disponibles contra leptospirosis bovina no incluyen esta variante autóctona. En este trabajo evaluamos la capacidad infectiva e inmunogénica de la variante autóctona de L. interrogans Pomona Kennewicki aislada de bovinos, en un modelo murino de infección sub-letal y crónica utilizando una vía de infección natural. Los resultados obtenidos demuestran la capacidad infectiva, de diseminación sanguínea, colonización del parénquima renal e inmunogenicidad de este aislamiento autóctono validando así su potencial uso como antígeno de interés a ser incluido en la formulación de vacunas anti-leptospira de uso veterinario en Uruguay y la región. --------------- SUMMARY: Leptospira interrogans serogroup Pomona serovar Kennewicki is one of the most frequently isolated variants in cattle in Uruguay. Currently available vaccine against bovine leptospirosis do not include this autochthonous strain. In this work we evaluated the infective and immunogenic capacity of the autochthonous variant of L.
interrogans Pomona Kennewicki isolated from bovines, in a chronic sublethal murine model using a natural route of infection. The results demonstrate the infective capacity, blood dissemination, colonization of the renal parenchyma and immunogenicity of this autochthonous isolate, thus validating its potential use as an antigen of int... Presentar Todo |
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Palabras claves : |
PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA. |
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Thesagro : |
BOVINOS DE CARNE; LEPTOSPIRA. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17132/1/Ciuffo-C.-et.al-Buiatria-Paysandu-2022.pdf
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Marc : |
LEADER 02587nam a2200241 a 4500
001 1064118
005 2023-05-11
008 2022 bl uuuu u01u1 u #d
100 1 $aCIUFFO, C.
245 $aEstudio y caracterización de una variante autóctona de Leptospira para su potencial uso como antígeno en vacunas de uso veterinario.$h[electronic resource]
260 $aIn: Jornadas Uruguayas de Buiatría, 49., 2022. Paysandú, Uruguay: Centro Médico Veterinario de Paysandú; Filial de la Sociedad de Medicina Veterinaria del Uruguaya; Sociedad Uruguaya de Buiatría, 9 al 11 Junio 2022. p.192-194.$c2022
300 $ap.192-194.
520 $aRESUMEN: En Uruguay, Leptospira interrogans serogrupo Pomona serovar Kennewicki es una de las variantes más frecuentemente aislada en bovinos. Las vacunas actualmente disponibles contra leptospirosis bovina no incluyen esta variante autóctona. En este trabajo evaluamos la capacidad infectiva e inmunogénica de la variante autóctona de L. interrogans Pomona Kennewicki aislada de bovinos, en un modelo murino de infección sub-letal y crónica utilizando una vía de infección natural. Los resultados obtenidos demuestran la capacidad infectiva, de diseminación sanguínea, colonización del parénquima renal e inmunogenicidad de este aislamiento autóctono validando así su potencial uso como antígeno de interés a ser incluido en la formulación de vacunas anti-leptospira de uso veterinario en Uruguay y la región. --------------- SUMMARY: Leptospira interrogans serogroup Pomona serovar Kennewicki is one of the most frequently isolated variants in cattle in Uruguay. Currently available vaccine against bovine leptospirosis do not include this autochthonous strain. In this work we evaluated the infective and immunogenic capacity of the autochthonous variant of L. interrogans Pomona Kennewicki isolated from bovines, in a chronic sublethal murine model using a natural route of infection. The results demonstrate the infective capacity, blood dissemination, colonization of the renal parenchyma and immunogenicity of this autochthonous isolate, thus validating its potential use as an antigen of interest to be included in the formulation of anti-leptospira vaccines for veterinary use in Uruguay and the region.
650 $aBOVINOS DE CARNE
650 $aLEPTOSPIRA
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
700 1 $aARÉVALO, A.P.
700 1 $aGARCIA, F.
700 1 $aPERDOMO, T.
700 1 $aCRISPO, M.
700 1 $aGIANNITTI, F.
700 1 $aBUSCHIAZZO, A.
700 1 $aZARANTONELLI, L.
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Registro original : |
INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
03/12/2020 |
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Actualizado : |
03/12/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
-- - -- |
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Autor : |
LLANES ALVAREZ, Y.; PEÑA BÁRZAGA, I.; BATISTA-LE RIVEREND, L.; PACHECO, R.; ZAMORA RODRÍGUEZ, V.; BENÍTEZ-GALEANO, M.J.; RIVAS, F.; BERTALMIO, A.; HERNÁNDEZ RODRÍGUEZ, L. |
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Afiliación : |
YILIAN LLANES-ALVAREZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; INÉS PEÑA-BÁRZAGA, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; LOCHY BATISTA-LE RIVEREND, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; RONAL PACHECO, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, México.; VICTORIA ZAMORA-RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; MARÍA JOSÉ BENÍTEZ-GALEANO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Laboratorio de Virología Molecular, Universidad de La República, Uruguay.; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LESTER HERNÁNDEZ RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba. INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Prevalence of mild citrus tristeza virus isolates of the T30 genotype in Cuban commercial citrus fields after the dissemination of huanglongbing. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Crop Protection, 2021, v. 140, art. 105422. DOI: https://doi.org/10.1016/j.cropro.2020.105422 |
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Páginas : |
11 p. |
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DOI : |
10.1016/j.cropro.2020.105422 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 5 May 2020; Received in revised form 8 October 2020; Accepted 12 October 2020; Available online 23 October 2020. |
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Contenido : |
Huanglongbing (HLB) disease detection in Cuba in 2006 led to a turn in citrus management in the country, including the production of certified budwood in protected nurseries, eradication of symptomatic plants and old orchards, and chemical control of the vector Diaphorina citri. This dramatic shift in citrus farming could have affected the incidence and composition of citrus tristeza virus (CTV) populations. This work aimed to determine CTV incidence and characterize viral isolates from the main Cuban citrus-producing areas. A survey throughout the country was carried out which resulted in the collection of 27 CTV isolates. Results showed a decrease of CTV incidence in western and central areas of the country, but an increase in the East, compared to previous reports. Bioindexing showed that most of the isolates induced none or mild symptoms. Serological characterization using seven different CTV specific monoclonal antibodies (MAb) showed heterogeneity among the 27 isolates. Six out of twenty-seven were MCA13-reactive, a MAb associated to the presence of severe CTV isolates. Nevertheless, sequence comparison of the p20, p23 and p25 genes evidenced a high nucleotide sequence identity among isolates, and according to the phylogenetic analyses, all the sequences belonged to the CTV T30 genotype. Thus, our results showed a decrease in the CTV incidence in the surveyed areas and the broad dissemination of mild CTV isolates of the T30 genotype, which could be partially explained by the shift in the management programs implemented for CTV and HLB control in Cuba. MenosHuanglongbing (HLB) disease detection in Cuba in 2006 led to a turn in citrus management in the country, including the production of certified budwood in protected nurseries, eradication of symptomatic plants and old orchards, and chemical control of the vector Diaphorina citri. This dramatic shift in citrus farming could have affected the incidence and composition of citrus tristeza virus (CTV) populations. This work aimed to determine CTV incidence and characterize viral isolates from the main Cuban citrus-producing areas. A survey throughout the country was carried out which resulted in the collection of 27 CTV isolates. Results showed a decrease of CTV incidence in western and central areas of the country, but an increase in the East, compared to previous reports. Bioindexing showed that most of the isolates induced none or mild symptoms. Serological characterization using seven different CTV specific monoclonal antibodies (MAb) showed heterogeneity among the 27 isolates. Six out of twenty-seven were MCA13-reactive, a MAb associated to the presence of severe CTV isolates. Nevertheless, sequence comparison of the p20, p23 and p25 genes evidenced a high nucleotide sequence identity among isolates, and according to the phylogenetic analyses, all the sequences belonged to the CTV T30 genotype. Thus, our results showed a decrease in the CTV incidence in the surveyed areas and the broad dissemination of mild CTV isolates of the T30 genotype, which could be partially explained ... Presentar Todo |
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Palabras claves : |
BIOLOGICAL CHARACTERIZATION; CLOSTEROVIRIDAE; CTV; GENOTYPING; MCA13; SEROLOGICAL CHARACTERIZATION. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 02768naa a2200325 a 4500
001 1061537
005 2020-12-03
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1016/j.cropro.2020.105422$2DOI
100 1 $aLLANES ALVAREZ, Y.
245 $aPrevalence of mild citrus tristeza virus isolates of the T30 genotype in Cuban commercial citrus fields after the dissemination of huanglongbing.$h[electronic resource]
260 $c2020
300 $a11 p.
500 $aArticle history: Received 5 May 2020; Received in revised form 8 October 2020; Accepted 12 October 2020; Available online 23 October 2020.
520 $aHuanglongbing (HLB) disease detection in Cuba in 2006 led to a turn in citrus management in the country, including the production of certified budwood in protected nurseries, eradication of symptomatic plants and old orchards, and chemical control of the vector Diaphorina citri. This dramatic shift in citrus farming could have affected the incidence and composition of citrus tristeza virus (CTV) populations. This work aimed to determine CTV incidence and characterize viral isolates from the main Cuban citrus-producing areas. A survey throughout the country was carried out which resulted in the collection of 27 CTV isolates. Results showed a decrease of CTV incidence in western and central areas of the country, but an increase in the East, compared to previous reports. Bioindexing showed that most of the isolates induced none or mild symptoms. Serological characterization using seven different CTV specific monoclonal antibodies (MAb) showed heterogeneity among the 27 isolates. Six out of twenty-seven were MCA13-reactive, a MAb associated to the presence of severe CTV isolates. Nevertheless, sequence comparison of the p20, p23 and p25 genes evidenced a high nucleotide sequence identity among isolates, and according to the phylogenetic analyses, all the sequences belonged to the CTV T30 genotype. Thus, our results showed a decrease in the CTV incidence in the surveyed areas and the broad dissemination of mild CTV isolates of the T30 genotype, which could be partially explained by the shift in the management programs implemented for CTV and HLB control in Cuba.
653 $aBIOLOGICAL CHARACTERIZATION
653 $aCLOSTEROVIRIDAE
653 $aCTV
653 $aGENOTYPING
653 $aMCA13
653 $aSEROLOGICAL CHARACTERIZATION
700 1 $aPEÑA BÁRZAGA, I.
700 1 $aBATISTA-LE RIVEREND, L.
700 1 $aPACHECO, R.
700 1 $aZAMORA RODRÍGUEZ, V.
700 1 $aBENÍTEZ-GALEANO, M.J.
700 1 $aRIVAS, F.
700 1 $aBERTALMIO, A.
700 1 $aHERNÁNDEZ RODRÍGUEZ, L.
773 $tCrop Protection, 2021$gv. 140, art. 105422. DOI: https://doi.org/10.1016/j.cropro.2020.105422
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